Digested mRNA Analysis of Sequence, 5’ Cap, and PolyA Tail
Comprehensive mRNA Characterization: Sequence, Cap Structures, and PolyA Tails in One Workflow
Webinar Summary
Discover a unified approach to mRNA PQA analysis with LC-MS/MS and advanced data processing in Byos. This webinar will showcase how enzymatic digestion, combined with ion-pair reversed-phase separation, enables in-depth profiling of mRNA sequence, 5’ cap structures, and PolyA tails. Using real-world data from eGFP mRNA, we’ll
demonstrate how to tackle isomer resolution, quantify capping efficiency, and deconvolute PolyA length variants—all within a streamlined, high-coverage workflow.
Key Learning Points
- Learn how digested oligonucleotide mapping provides >99% sequence coverage for mRNA, even in the presence of isomeric oligos.
- Understand how to quantify 5’ cap variants and intermediates using Byos software and robust MS/MS scoring techniques.
- See how PolyA tail deconvolution and mass matching reveal length distributions with LC-resolved detail for high-confidence PQA assessment.
Recorded April
2025
Recommended Resources
WEBINARMS Analysis of Intact Oligonucleotides
Explore advanced workflows for oligonucleotide analysis, focusing on fragmentation and MS2 annotation.
VIDEO SERIESWorking with Oligonucleotide Workflows
Learn how to automate oligonucleotide data processing, handle raw files, define sequences, and generate custom reports.
VIDEO SERIESWorking with Digested Oligo Workflows
Learn Byos Digested Oligonucleotide workflows, modification setup, investigation tools, and custom report creation.
PUBLICATIONAutomated LCMS Analysis of Oligonucleotide Samples
Improve your organization’s efficiency and capability with automated LCMS analysis of synthetic oligos.