ASMS 2025 - Comprehensive isoform annotation and quantitation in DIA data
Advanced DIA workflow within Byos® improves sensitivity, accuracy, and consistency in proteomic analysis
About this Poster
This poster introduces a novel, high-performance search engine designed to enhance isoform annotation and quantitation in Data Independent Acquisition (DIA) proteomics. Integrated into the Byos® platform, the new engine improves sensitivity, reduces quantitation variability, and increases match confidence—particularly in complex datasets with overlapping isotope signals. Benchmarking against Byonic™, the study highlights improvements in identification rates, scoring fidelity, and consistency across replicates.
Key Learnings:
- Discover how the new search engine recovers more confident identifications in complex DIA data using multi-isotope profiling.
- Learn how enhanced elution boundary detection improves quantitation precision and reduces coefficient of variation.
- Understand how integrated scoring across MS2 scans reduces noise and improves modification site localization
