How to Unlock the Power of Peptide Analysis with Byos
Master advanced peptide analysis with Byos. Learn expert techniques for PTM searches, glycopeptide characterization, and quantification workflows.
About this EBook
Peptide analysis sits at the crossroads of biology and technology. As therapeutic proteins become more complex and analytical standards rise, scientists face mounting pressure to identify, quantify, and validate every peptide modification with precision.
Yet the challenges are formidable: incomplete MS2 coverage, co-eluting species, overlapping isotopes, heterogeneous glycoforms, and post-translational modifications that defy simple rules. This is where Byos transforms the landscape.
This eBook brings together six practical guides that collectively form a comprehensive roadmap for advanced peptide analysis using Byos. From foundational search strategies to advanced quantification and validation workflows, you'll learn how to unlock the full power of peptide analysis without leaving the Byos environment.
What You'll Learn
Master Advanced Peptide Analysis Techniques
1. Optimizing Post-Translational Modification Searches in Byos
Learn how to configure Byos for faster, more accurate PTM searches using Modification Fine Control. Understand the difference between common and rare modifications and how to adjust parameters for optimal performance.
2. Accurate Quantification of Asparagine Deamidation in Protein Therapeutics
Discover how to identify and quantify one of the most critical PTMs in biotherapeutic development, including techniques for distinguishing aspartic acid and iso-aspartic acid forms.
3. How to Effectively Search N-Linked Glycopeptides with Byos
Configure Byos for accurate and efficient N-linked glycopeptide identification at the composition level, with best practices for database selection and search optimization.
4. How to Perform Efficient O-Linked Glycopeptide Analysis
Practical strategies for managing O-linked glycopeptide complexity while maintaining confidence and efficiency, including Delta Mod Score validation techniques.
5. How to Analyze Multiple Digest Peptide Projects in Byos
Maximize sequence coverage and PTM validation through multi-enzyme workflows, learning how to configure parallel MS/MS identification nodes for different digestion conditions.
6. How to Use In-Silico Peptides for MS1 Quantification
Extend quantification beyond MS2 coverage using theoretical peptides and MS1-level data to capture complete quantitative information from your LC-MS runs.