Progressive Deconvolution for Robust Intact MS Analysis
Beyond Conventional Deconvolution: Tackling Complex Biologics and ADCs
Progressive Deconvolution: Unlocking Low-Abundance Proteoforms and Improving Dynamic Range
This best-in-class deconvolution algorithm robustly addresses the size and heterogeneity of modern biotherapeutics, where older MS-based techniques are insufficient.
- Maximum Dynamic Range:Progressive Deconvolution yields many additional high-confidence IDs compared to conventional methods. This massive improvement is due to a drastically higher validated dynamic range (e.g., increasing 14.7 to 42.5 in one study).
- Superior Sensitivity and Fidelity:This method provides a robust and sensitive feature detection tool. It successfully reveals low-abundance forms and assures that lower abundance intact species are detected with high confidence, thus faithfully rendering all proteoforms.
- Isomer Resolution:Utilizing an iterative approach on sequential time ranges, progressive deconvolution is able to distinguish critical isomeric forms based on their differential elution patterns.
- Essential for Complex Biologics:The tool is crucial for accurate characterization of highly complex samples, including providing robust Drug-to-Antibody Ratio (DAR) calculation for ADCs like Kadcyla and resolving hundreds of individual glycoforms.
