Comprehensive Characterization of Disulfide Linkages in a Cysteine-Engineered Bispecific Antibody Using EThcD-on a modified Orbitrap Hybrid Mass Spectrometry

Disulfide Peptide Analysis: High-Confidence Structural Confirmation

This technology ensures the accurate characterization of complex disulfide peptides, a critical requirement in biotherapeutic development, by leveraging specialized fragmentation data.

• Advanced Fragmentation Mapping:Our software efficiently processes complex MS/MS data generated by ETD, EthcD, and HCD. The data provides comprehensive fragment coverage, successfully mapping ions such as b, y, c, and z fragment types.
• Performance Comparison:Visual data compares the percentage of disulfide peptides identified using ETD, EthcD, and HCD, demonstrating the relative efficacy of these different fragmentation techniques.
• Detailed Sequence Verification:Detailed spectral analysis confirms sequence assignment for specific peptides, including PGCVP and GGGSGGSGGCPP. This process provides high-confidence structural confirmation based on specific fragment ions like z9(2), y10(2), and c3z8(2).
• Flexible Workflow Support:The analysis supports various digestion approaches, including both KPA Semi-Specific Digestion and Non-specific Digestion, providing validated peptide hits across different amino acid compositions.