ASMS 2026: Progressive Deconvolution for Comprehensive Analysis of Erythropoietin
Detecting low-abundance EPO glycoforms with Byos Progressive Deconvolution and Genovis enzymatic sample prep
About this Poster
Erythropoietin is roughly 40% glycan by weight — three N-linked sites, one O-linked, varying sialylation, and a long tail of low-abundance glycoforms that traditional intact-mass deconvolution can flatten or lose. This poster, a Protein Metrics and Genovis collaboration, shows how Byos Progressive Deconvolution combined with targeted enzymatic sample prep (SialEXO, OglyZOR, and PNGase F) extends the validated dynamic range of intact MS far enough to detect and confidently assign EPO glycoforms that Trace Peak deconvolution misses — including an H(1)N(1) species visible only after Progressive Deconvolution with minimum-consecutive-slices filtering.
Key Learnings:
- See how Progressive Deconvolution's mass-time matrix and multi-dimensional segmentation expose low-abundance EPO glycoforms that conventional Trace Peak deconvolution misses.
- Compare deconvolved spectra across four sample treatments — untreated, PNGase F, SialEXO, and combined — to understand how each enzyme reshapes the intact-mass landscape.
- Learn how mass XICs from Progressive Deconvolution enable AUC-based relative quantitation that complements traditional intensity-based methods, with comparable but more complete coverage.
- Understand the minimum-consecutive-slices filter that lets Byos confirm low-abundance assignments with high confidence, demonstrated on an H(1)N(1) species visible only after Progressive Deconvolution.
In collaboration with Genovis (Lund, Sweden) — SialEXO®, OglyZOR®, and other enzymatic tools used for sample preparation.