ASMS 2026: Mass Spec Software to Aid Data Interpretation at the Speed of Biotherapeutics Development
Automated raw-data processing, intelligent triage, and plate heatmaps for 96-well CHO clone screening, with Johnson & Johnson Innovative Medicine
About this Poster
The rapid expansion of complex biotherapeutic formats — multispecific antibodies, antibody-drug conjugates, scFvs, engineered fragments — has increased both the complexity and the volume of mass spectrometry data generated during discovery and development. Manual review of every spectrum stops scaling. This poster, from Johnson & Johnson Innovative Medicine in collaboration with Protein Metrics, demonstrates an advanced vendor-neutral MS software platform that combines automated raw-data processing, interactive visualization, ML- and logic-assisted triage, traffic-light quality indicators, plate-based heatmaps for clone ranking, and automated report generation. Applied to a 96-well CHO screening dataset for an anti-RSV scFv-Fc × Fab bispecific, the workflow let lab managers assess dataset-level quality without ever opening a raw spectrum.
Key Learnings:
- See how automated visualization and intelligent triage turn 96-well CHO clone screening data into rapid, decision-oriented summaries.
- Understand how traffic-light triage (Green / Yellow / Red) flags high-quality clones and routes ambiguous cases to expert review — reducing manual burden by hours per dataset.
- Compare clones on correct molecular weight, desired bispecific antibody formation, balanced chain expression, and low off-target species — all in one plate heatmap.
- Learn how plate-based visualization supports rapid cherry-picking of top clones during cell line development for complex multichain biologics.
Work led by Johnson & Johnson Innovative Medicine (Spring House, PA) in collaboration with Protein Metrics (Boston, MA).