ASMS 2026: A Sweet Solution for Mass Spectrometric Glycan Analysis
Automated glycan grouping in Byos streamlines glycan analysis from released-glycan HILIC-FLR to peptide-level MAM workflows
About this Poster
Glycans significantly influence the stability, activity, and immunogenicity of biologics — and getting their analysis right matters especially for biosimilars and multi-glycan-site molecules where HILIC-FLR alone doesn't provide enough information. This poster, from Just-Evotec Biologics in collaboration with Protein Metrics, demonstrates a glycan grouping tool that streamlines mass-spectrometry-based glycan analysis — automating tedious manual glycan categorisation, enabling clean visualization across days of culture and bioreactors, and bridging data from released-glycan HILIC-FLR with peptide-level multi-attribute method (MAM) workflows.
Key Learnings:
- See how automated glycan grouping transforms complex MAM data into intuitive, comparable visualizations across 12 bioreactors and 3 days of culture.
- Compare released-glycan HILIC-FLR (overall glycan profile and diversity) with peptide-level MAM (occupancy and site-specific information) — and understand when each is the right tool.
- Learn how the Byos glycan grouping tool reduces manual categorisation effort while keeping the underlying glycoform-level detail accessible.
- Discover how Just-Evotec Biologics applied the workflow to track sialylation and other glycan groups across a multi-site, multi-bioreactor process development study.
Just-Evotec Biologics (Seattle, WA) led the experimental work, in collaboration with Protein Metrics on the glycan grouping tool.