Quick Guide to Charge Variant Analysis

Charge Variant Analysis with Reconstruction Workflow

A new multi-node workflow using Byonic, Byologic, and Byomap has been added to Byos to support Charge Variant Analysis with Reconstruction.

Why icIEF Charge Variant Reconstruction?

• Root cause analysis without fractionation — Identify which PTMs drive charge heterogeneity using existing peptide mapping data, eliminating days of fraction collection and analysis
• Connect orthogonal data — Bridge cIEF profiles with peptide-level PTM quantitation to answer: which modifications cause which peaks?
• Decode complex charge profiles — Bispecifics and novel formats produce multi-peak electropherograms that defy simple interpretation. Reconstruction attributes each peak to specific PTM combinations
• Clone screening at scale — Enable PTM-charge attribution across hundreds of cell line candidates without additional experiments
• Hypothesis-driven investigation — Generate testable predictions about charge variant origins before committing to targeted studies
• Regulatory-ready insight — Provide mechanistic explanation of charge heterogeneity backed by peptide-level evidence for comparability and filing support

Create a Workflow

Within Project Creation within the workflow, users should add an MS file and pI file in their own row entry, as well as the relevant FASTA file(s). Within the processing node, check/change the following settings: Make sure the Sample Names for the Byonic and Byologic nodes are reading the proper MS file.

Attach the report template Blgc_Reconstruction_export.rptc within the Byologic node and create project.

There should be two projects automatically created with the same filename, one .blgc and one .bmap. Both projects should automatically generate reports. The Report for the blgc project should be the one that was generated with the defined report template.