Improve MS Quality of Intact Proteins in Complex Mixtures
Webinar exploring how combining ion-mobility with Protein Metrics HRIM software improves data processing and enhances mass spectral analysis accuracy.
About This Webinar
Improving Mass Spectral Quality of Intact Proteins in Complex Mixtures
Presented by Matthew Crawford, Senior Scientist, Rilas Technologies in October 2024
Generating intact protein charge envelopes during mass spectrometry requires careful sample prep and excludes several reagents often used in biological assays. These reagents typically inhibit non-specific interactions between analyte molecules and testing vessels (plates, vials, etc.), while maintaining environments favorable to protein folding. Common examples include salts (NaCl, KCl, MgCl2, phosphate) and detergents (NP-40, SDS, Tween-20), which can suppress signals or complicate spectra.
MOBILion Systems developed a high-resolution ion-mobility system that separates compounds by their collisional cross section (CCS) in the gas phase. This technology separates analytes from contaminants post-ionization, simplifying intact protein analysis. In this seminar, we’ll explore how combining ion-mobility with Protein Metrics HRIM software improves data processing and enhances mass spectral analysis accuracy.
From The Webinar
On software capabilities: "PMI has been indispensable for this particular workflow because HRIM serves as a true fourth dimension in data acquisition for this analysis." 19:41
On user-friendliness: "Their packages are about as user friendly as someone could ask for with these complicated datasets." 20:32
On automated workflows: "it also enables development of automated workflows with higher levels of fidelity as HRIM is able to filter out noise." 20:43
On polygonal extraction: "Protein Metrics has been really pushing the envelope for this. And they've developed a polygonal extraction system." 32:00