Smarter Digestion, Deeper Insight: Improving LC-MS of Therapeutic mRNA
Combining optimized digestion and CE tuning for better sequence and cap detection.
About This Webinar
Therapeutic mRNAs hold enormous promise—but ensuring their quality and structural integrity is no small task. In this webinar, scientists from ProtaGene and Shimadzu present a practical LC-MS workflow to address these challenges head-on. By using tailored enzymatic digestion, dynamic collision energy optimization, and Protein Metrics software for confident oligo identification, the team achieved high sequence coverage and reliable detection of 5' cap structures. Whether you're working with vaccines or gene therapies, this session offers valuable insights into next-gen mRNA analytics.
What You Will Learn
- Tailored enzymatic digestion improves sequence coverage and reproducibility.
- Dynamic CE optimization reduces noise and improves fragmentation clarity.
- Cap structures and modifications can be confidently verified by LC-MS.
- Protein Metrics software enables reliable oligo matching and streamlined analysis.
Our Speaker
Pierre-Alexander Mücke, Ph.D., is a scientist at ProtaGene GmbH with deep expertise in mass spectrometry-based characterization of protein and oligonucleotide therapeutics. As part of ProtaGene’s dedicated MS group, he focuses on sequence verification, PTM analysis, disulfide bridge elucidation, and the development of advanced analytical methods for oligonucleotide-based drugs. Dr. Mücke holds a Ph.D. in biochemistry from the University of Greifswald, with academic training in Düsseldorf and Stockholm. Prior to his current role, he gained experience in both regulatory affairs and academic research, including positions at Cheplapharm Arzneimittel GmbH and Ruhr University Bochum.
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