Comprehensive ADC Analysis via Automated MS Workflows

Webinar Summary

From Fractionation to MS: Full ADC Characterization

Antibody-drug conjugates (ADCs) present complex analytical challenges due to their structural heterogeneity. This webinar explores how automated sample fractionation, magnetic bead-based digestion, and nanoflow LC-MS/MS are combined into a seamless workflow for in-depth ADC characterization. Case studies demonstrate how the approach enables confident localization of conjugation sites, identification of disulfide scrambling, and insight into product heterogeneity. The session also highlights key lessons learned in adopting nanoflow LC-MS, including sensitivity gains and oxidation artifacts.

Our speaker, Dan Bach Kristensen, Scientific Director at Symphogen, brings over two decades of deep expertise in analytical protein chemistry, with extensive hands-on experience in mass spectrometry, chromatography, and biopharmaceutical development across a wide range of therapeutic areas.

Learning Points

• Learn how automated chromatographic fractionation simplifies ADC sample preparation.
• Understand the integration of robotic digestion and magnetic beads for consistent peptide generation.
• Discover how nanoflow LC-MS/MS enhances sensitivity for low-abundance ADC species.
• Examine real-world case studies mapping conjugation variability and disulfide scrambling