Comprehensive ADC Analysis via Automated MS Workflows
Automated workflows for analyzing antibody-drug conjugates, from fractionation and digestion to high-sensitivity nanoflow LC-MS/MS.
Webinar Summary
From Fractionation to MS: Full ADC Characterization
Antibody-drug conjugates (ADCs) present complex analytical challenges due to their structural heterogeneity. This webinar explores how automated sample fractionation, magnetic bead-based digestion, and nanoflow LC-MS/MS are combined into a seamless workflow for in-depth ADC characterization. Case studies demonstrate how the approach enables confident localization of conjugation sites, identification of disulfide scrambling, and insight into product heterogeneity. The session also highlights key lessons learned in adopting nanoflow LC-MS, including sensitivity gains and oxidation artifacts.
Our speaker, Dan Bach Kristensen, Scientific Director at Symphogen, brings over two decades of deep expertise in analytical protein chemistry, with extensive hands-on experience in mass spectrometry, chromatography, and biopharmaceutical development across a wide range of therapeutic areas.
Learning Points
- Learn how automated chromatographic fractionation simplifies ADC sample preparation.
- Understand the integration of robotic digestion and magnetic beads for consistent peptide generation.
- Discover how nanoflow LC-MS/MS enhances sensitivity for low-abundance ADC species.
- Examine real-world case studies mapping conjugation variability and disulfide scrambling
From The Webinar
On system integration: "the important point here is that these systems talk together. All the data acquired in Chromelion is automatically linked and synchronized into Byosphere. So there's no manual manipulation of the data. Everything is automatically synchronized between the systems and accessible in both systems." 10:40
On workflow power: "So all in all, super powerful technologies that can really help you to support the process development for your biopharmaceutical projects." 39:14
On ADC characterization capabilities: "So really a site specific information that you can get by combining these intact mass and peptide mass workflows." 32:03
On peptide mapping confirmation: "So the peptide mapping really confirms that we do see issues in the hinge region, and that is giving us this highly complicated pattern that we observe for this product." 35:47
On deep insights: "when you combine characterization of these fractions by intact mass and peptide mapping, you get really deep insight into what is going on at the amino acid level and what kind of modifications are responsible for the purity profile you see in your impurity methods." 38:16